Survey of echinococcoses in southeastern Qinghai Province, China, and serodiagnostic insights of recombinant Echinococcus granulosus antigen B isoforms

Immunodiagnostic Value of Echinococcus Granulosus Recombinant B8/1 Subunit of Antigen B

Background: Cystic echinococcosis (CE), as a chronic parasitic disease, is a major health problem in many countries. The performance of the currently available serodiagnostic tests for the diagnosis of CE is unsatisfactory. Objective: The current study aimed at sub-cloning a gene, encoding the B8/1 subunit of antigen B (AgB) from Echinococcus granulosus, using gene optimization for the immunodi...

Isolation and purification of Echinococcus granulosus antigen B from hydatid cyst fluid using three different methods

Hydatid cyst, the larval stage of cestodes Echinococcus spp., is recognized as a zoonotic infection in the world. The World Health Organization (WHO) has recently classified echinococcosis in a group of neglected tropical diseases.The prevalence of Echinococcus granulosus infection is high in Iran due to the presence of various intermediate hosts in this country. Considering the rising trend of...

subcloning and expression of recombinant echinococcus granulosus antigen b, in pqe-30 expression vector

background: echinococcosis or hydatid disease is a zoonotic infection caused by larval (metacestode) stages of cestodes belonging to the genus echinococcus, family taeniidae . we aimed to subclone antigen b gene in pqe-30 plasmid, its expression, and purification. methods: we subcloned hi gene into pqe-30 expression vector. the recombinant vector was transformed into e. coli , m15 and mass cult...

Immunodiagnostic Value of Echinococcus Granulosus Recombinant B8/1 Subunit of Antigen B.

BACKGROUND Cystic echinococcosis (CE), as a chronic parasitic disease, is a major health problem in many countries. The performance of the currently available serodiagnostic tests for the diagnosis of CE is unsatisfactory. OBJECTIVE The current study aimed at sub-cloning a gene, encoding the B8/1 subunit of antigen B (AgB) from Echinococcus granulosus, using gene optimization for the immunodi...

Study of Echinococcus granulosus and Echinococcus multilocularis infections in canidiae in Ardabile province of Iran